Fig. 7

Inhibition of T cell proliferation by Gal-9 high-expressing ERCs is associated with Lck/ZAP70/LAT pathway. The naïve CD4⁺ T cells in the mouse spleen were collected by magnetic bead, and then the harvested CD4⁺ T cells were co-cultured with unmodified ERCs or Gal-9 high-expressing ERCs for 6 days. A CD4⁺ T cell proliferation was determined by CCK-8 assay. B The protein expressions and phosphorylation form protein expressions of Lck, ZAP-70, LAT were detected by western blot. The differences between groups were calculated by using ANOVA. *p < 0.05, **p < 0.01. Abbreviations: Lck lymphocyte-specific protein tyrosine kinase, ZAP-70 zeta-chain-associated protein kinase 70, LAT linker for activation of T cells