Fig. 2
From: Noncanonical Wnt5a signaling regulates tendon stem/progenitor cells senescence
Wnt5a knockdown attenuates TSPCs senescence. a Heatmap of aging-related genes expressions in aged and Wnt5a-knockdown aged TSPCs. b, c β-gal staining for the senescent cells in young, aged and Wnt5a-knockdown aged TSPCs. Scale bars: 100 μm. d Western blotting for p16INK4A protein levels in young, aged and aged Wnt5a-knockdown TSPCs. e Flow cytometry analysis was used to measure the cell cycle distribution of TSPCs. f, g Representative distribution of tubulin in young, aged and aged Wnt5a-knockdown TSPCs was investigated by immunofluorescence. Scale bar: 20 μm. Values represent the mean ± SD. *p < 0.05, significantly different from the young or aged group