Fig. 4
From: Noncanonical Wnt5a signaling regulates tendon stem/progenitor cells senescence
Wnt5a activates JAK–STAT signaling pathway in TSPCs senescence. a The GSEA KEGG analysis revealed the top 10 significantly enriched signaling pathways in Wnt5a-knockdown aged TSPCs. b GSEA plots showed a gene set related to JAK–STAT pathway. c Heatmap of relative expression of the genes involved in the JAK–STAT signaling pathways in aged and Wnt5a-knockdown aged TSPCs. d Western blotting for the p-JAK2, JAK2, p-STAT3 and STAT3 protein levels in young, aged and Wnt5a-knockdown aged TSPCs. e Aged TSPCs were transfected with or without Wnt5a shRNA and then stimulated with IFN-γ at 100 ng/ml for 8 h. The protein levels of p-JAK2, JAK2, p-STAT3 and STAT3 were investigated by western blotting. f Western blotting for the p16INK4A protein levels in aged TSPCs upon Wnt5a shRNA and/or IFN-γ treatment for 8 h. g, h β-gal staining in aged TSPCs upon Wnt5a shRNA and/or IFN-γ (100 ng/ml, 24 h) treatment. Scale bars: 100 μm. i–k Relative mRNA levels of SASP genes (IL6, Ereg and Cxcl5) in aged TSPCs upon Wnt5a shRNA and/or IFN-γ (100 ng/ml, 8 h) treatment. Values represent the mean ± SD. *p < 0.05, significantly different from the young or aged group