Fig. 5
From: Noncanonical Wnt5a signaling regulates tendon stem/progenitor cells senescence
Wnt5a activates JAK–STAT signaling pathway through its co-receptor Ror2. a Young TSPCs were transfected with or without Ror2-siRNA and then stimulated with Wnt5a at 100 ng/ml for 16 h. The expressions of p-JAK2, JAK2, p-STAT3 and STAT3 were investigated by western blotting. b Western blotting for the p16INK4A protein levels in young TSPCs upon Ror2-siRNA and/or Wnt5a (100 ng/ml, 16 h) treatment. c, d β-gal staining for the senescent cells in young TSPCs upon Ror2-siRNA and/or Wnt5a (100 ng/ml, 36 h) treatment. Scale bars: 200 μm. e–g qRT-PCR for IL6, Ereg and Cxcl5 mRNA levels in young, young TSPCs upon Ror2-siRNA and/or Wnt5a (100 ng/ml, 16 h) treatment. Values represent the mean ± SD. *p < 0.05, significantly different from the young or aged group