Fig. 1
Comparison of conditioned media from single and pooled donors of amniotic membrane derivatives on PBMC proliferation. Allogeneic PBMC (1 × 105) were stimulated with anti-CD3 antibody in the presence of 100 µL (50%) or 50 µL (25%) of conditioned medium (CM) from hAMSC at passage 0 (CM-hAMSC p0) or passage 2 (CM-hAMSC p2), or CM from hAM collected 2 or 5 days after culture (CM-hAM D2 and CM hAM D5, respectively). Results are expressed as percentage of cell proliferation. PBMC stimulated with anti-CD3 mAb constitute the positive control. CMs were either kept separately (A, single donors) or pooled (B) from multiple donors. Results are displayed as violin plots showing median (thick line), 25th and 75th quartiles (*p < 0.01, ***p < 0.001 versus control (PBMC + anti-CD3), A: N ≥ 7; B: N ≥ 4 individual experiments