Fig. 2

Effect of conditioned media from single and pooled donors of from amniotic membrane derivatives on Th1/Th2/Th17 polarization. Allogeneic PBMC were stimulated with anti-CD3 mAb and co-cultured with 100 µL (50%) or 50 µL (25%) of conditioned medium (CM) from hAMSC at passage 0 (CM-hAMSC p0) or passage 2 (CM-hAMSC p2), or CM from hAM collected 2 or 5 days after culture (CM-hAM D2 and CM hAM D5, respectively). CMs were either kept separately or pooled from multiple donors. Th1 (CD183+CD196−), Th1/Th17 (CD183+CD196+) and Th2 (CD183-CD196-CD194+) phenotype was evaluated. Results are displayed as violin plots showing median (thick line), 25th and 75th quartiles (*p < 0.05, **p < 0.01, ****p < 0.0001), N ≥ 3 individual experiments