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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: CM from intact hAM: an easily obtained product with relevant implications for translation in regenerative medicine

Fig. 3

Effect of conditioned media from single and pooled donors of amniotic membrane derivatives on cytotoxicity marker expression by (A) T lymphocytes and (B) NK cells. Allogeneic PBMC were incubated with anti-CD3 mAb in the presence of 100 µL (50%) or 50 µL (25%) of conditioned medium (CM) from hAMSC at passage 0 (CM-hAMSC p0) or passage 2 (CM-hAMSC p2), or CM from hAM collected 2 or 5 days after culture (CM-hAM D2 and CM hAM D5, respectively). CMs were either kept separately or pooled from multiple donors. The percentage of IFN-γ, granzyme B and perforin positive cells within the (A) CD4+, CD8+ T cell and (B) CD3-CD56+ NK cell populations was assessed by flow cytometry. Results are displayed as violin plots showing median (thick line), 25th and 75th quartiles (*p < 0.05, **p < 0.01, ***p < 0.001, versus control (PBMC + CD3), N ≥ 3 individual experiments

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