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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: CM from intact hAM: an easily obtained product with relevant implications for translation in regenerative medicine

Fig. 4

Comparison of lyophilized and cryopreserved CM on T-cell proliferation, Allogeneic PBMC were stimulated with anti-CD3 mAb (upper panel) or in mixed T lymphocyte reaction (MLR-T, bottom panel) in the presence of three different concentrations of cryopreserved or lyophilized CM, 100 µL (50%), 50 µL (25%) or 10 µL (5%), from hAMSC at passage 0 (CM-hAMSC p0) or passage 2 (CM-hAMSC p2), or CM from hAM collected at 5 days after culture (CM hAM D5). Results are represented as violin plots showing median (thick line), 25th and 75th quartiles (*p < 0.05, **p < 0.01, ****p < 0.0001 versus control PBMC + antiCD3 or T cells + PBMC*), N ≥ 3 individual experiments

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