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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Secretome of endothelial progenitor cells from stroke patients promotes endothelial barrier tightness and protects against hypoxia-induced vascular leakage

Fig. 4

scCM promotes endothelial barrier tightness. scCM reduced the permeability of CMECs, while it did not affect BLECs (a). scCM upregulated the expression of junctional proteins on CMECs (b). Heat map representing 23 genes upregulated by scCM on CMECs, most of them associated with the IFN pathway (c). scCM reduced the content of active β-catenin and claudin 3 (d) and downregulated mRNA expression of Wnt pathway targets APCDD1, Axin2, and CCND1 on CMECs (e). Co-incubation with Wnt inhibitor (AZ6102, 1 μmol/L) decreased barrier permeability of cells treated with scEBM (f). Data represent median (interquartile range) [b, d, and e] or mean ± SD [a and f] (n = 4–8). *P < 0.05vs scEBM CMECs

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