Fig. 5

OGD-induced vascular leakage in vitro is prevented by scCM. Treatment with scCM during reoxygenation (24 h) abolished the OGD-induced increase in permeability on CMECs (a). WB analysis revealed that scCM increased occludin, ZO-1, and tricellulin expression (b) and favoured their localization at cell junctions (c). scCM increased VEGFR2 activation, while it did not affect ERK1/2 or AKT activation (d). Besides, scCM upregulated VCAM-1, ICAM-1, and COX2 expression (d). Data represent median (interquartile range) [b and d] or mean ± SD [a] (n = 3–5). *P < 0.05 vs scEBM; $P < 0.05 vs R-OGD scEBM. Scale bar: 10 μm