Fig. 6

OGD-induced in vitro BBB leakage is prevented by scCM. Treatment with scCM during reoxygenation (24 h) abolished the OGD-induced increase in permeability on BLECs (a). WB analysis revealed that scCM increased claudin 5 expression (b) and favoured its localization at the cell junctions (c). scCM decreased VEGFR2 and ERK1/2 activation during reoxygenation (d). Moreover, scCM increased ICAM-1 expression in normoxic conditions but had no effect on BLECs exposed to OGD (d). Data represent median (interquartile range) [b and d] or mean ± SD [a] (n = 3–5). *P < 0.05 vs scEBM; $P < 0.5 vs R-OGD scEBM. Scale bar: 10 μm