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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Red blood cells and their releasates compromise bone marrow-derived human mesenchymal stem/stromal cell survival in vitro

Fig. 1

Fluorescent imaging of MSCs in HCTs at Day 1 (A–F), Day 3 (G–L) and quantitation of MSC viability Day 1–3 for HCT (M–O) and RBCrel (P–R). MSCs in culture were stained with calcein-AM (green, live) and DAPI (blue, dead) and imaged prior to harvesting for viability analysis. Day 1 (A). 0% HCT control (B). 2.5% HCT (C). 5% HCT (D). 10% HCT (E). 20% HCT, (E)(i). 20% HCT with fragmentation of nuclei (subpanel is enlarged, not to scale) (F). 40% HCT (arrow shows microparticle formation from MSC). Day 3 (G). 0% HCT control (H). 2.5% HCT (I). 5% HCT (J). 10% HCT (K). 20% HCT (L). 40% HCT, (L)(i). 40% HCT with RBC aggregate containing non-viable MSCs (subpanel is enlarged, not to scale). For all micrographs: Scale bar = 860 microns; Total magnification = 104x (excluding E)(i). and (L)(i).). Quantitation of viable MSCs via fluorescent microscopy in HCT and RBCrel conditions. Viable MSCs in HCT conditions at (M) Day 1, (N) Day 2 and (O) Day 3. Viable MSCs in RBCrel conditions at (P) Day 1, (Q) Day 2 and (R) Day 3. Error bars represent the standard deviation of the data set. Levels of significance: *p < 0.05, **p < 0.01

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