Fig. 2
Staining, gating and analysis of MSCs via CD90, AV and 7AAD. a Controls for apoptosis and necrosis via H2O2 and doxorubicin, respectively. b Untreated MSCs gated via FITC-isotype control for CD90 gating (histogram) and unlabeled for AV and 7AAD (complimenting the axes in “a”) for the following HCT and RBCrel data sets. c CD90-labeled MSC health status in HCT conditions Day 1–3. d CD90-labeled MSC health status in RBCrel conditions Day 1–3. Dot plot quadrants for c and d are identical to the labeled controls in (a) and are as follows: viable (AV-/7AAD-) are in the lower left quadrant, AV + /7AAD- are upper left quadrant, AV + /7AAD + upper right and AV-/7AAD + are lower right quadrant