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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Hair follicle-derived mesenchymal stem cells decrease alopecia areata mouse hair loss and reduce inflammation around the hair follicle

Fig. 2

Growth-suppression and MHC I expression upregulation of interferon (IFN)-γ treatment in the organ-cultured model. Mouse vibrissa follicles were isolated and cultured in HF medium with PBS or 100 IU mL−1 IFN-γ addition. A Mouse vibrissa follicles were photographed on day 0 and day 4. B Hair shaft growth was inhibited by interferon (IFN)-γ in organ-cultured mouse vibrissa follicles on the 4 days (n = 12/each group). C–E The mRNA transcript levels of MHC I, caspase1 and Ki67 in mouse vibrissa after 4 days of PBS or IFN-γ treatment measured by qRT-PCR, expression levels of those mRNA were normalized with GAPDH. F–H The protein expression levels of MHC I, caspase1 and Ki67 in mouse vibrissa after 4 days of PBS or IFN-γ treatment measured by western blotting. Relative protein expression levels were averaged from three groups of biology repeatedly and normalized with β-actin. The results were expressed as the Mean ± SD, *p < 0.05

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