Fig. 5

Immunoconfocal colocalization of SSEA1 (red color) with DAPI (blue color), and ET-1, ICAM-1, IL-1 beta, AP-2, BDNF, and VEGF (green color) in CCA artery wall. SSEA1 is not visible in the groups of before LED and LED alone but is strongly expressed after iPSCs treatment at 1 day and 7 days. ET-1 and IL-1 beta are rarely expressed before LED irradiation. However, they show strong expressions in LED alone group at 1 day and 7 days, and the expressions are reduced after iPSCs treatment. AP-2, BDNF and VEGF are lightly visible in before LED group and reduced in LED alone group at 1 day and 7 days. However, AP-2, BDNF and VEGF are increased after iPSCs treatment. ICAM-1 is not colocalized with SSEA1 due to the conflict of secondary antibodies. AP-2 = angiopoietin-2; BDNF = brain-derived neurotrophic factor; DAPI = 4’,6-diamidino-2-phenylindole; ET-1 = endothelin-1; ICAM-1 = intercellular adhesion molecule-1; IL-1 = interleukin 1 beta; iPSCs = mouse induced pleuripotent stem cells; LED = light-emitting diode; SSEA1 = stage-specific embryonic antigen 1; VEGF = vascular endothelial growth factor