Table 1 Primer Sequences (Reverse Transcription Polymerase Chain Reaction)
Type | Gene | Sequence (5’–3’) | Tm (℃) | Accession no |
|---|---|---|---|---|
Calcium channel | SERCA2b | F: 5’- CCAGTCGATTCTTACAGGTG -3’ R: 5’- GCGATGTTTGTGCCAGAAAA -3’ | 56 | NM_001110823.2 |
IP3R | F: 5’- GGAGCCTCTGGTGAAAACGA -3’ R: 5’- CACCCATGTTCCTCAGCAGT -3’ | 59 | NM_001007235.2 | |
GRP75 | F: 5’- TGATGCCAATGGGATTGTGC -3’ R: 5’- CTGCTTCAACACGTTCCTTC -3’ | 58 | NM_001100658.2 | |
CaM | F: 5’- GTGAGGCATTCCGAGTCTTT -3’ R: 5’- TCATCTGTTAGCTTTTCCCCG -3’ | 57 | NM_031969.3 | |
STIM1 | F: 5’- ACAAGCTTATCAGCGTGGAG -3’ R: 5’- ACTTCCGGAAAGTCTCCTCA -3’ | 57 | NM_001108496.2 | |
VDAC1 | F: 5’- AACAGTAACACTCGCTTTGG -3’ R: 5’- TTGACGTTCTTGCCATCCAG -3’ | 57 | NM_031353.2 | |
MCU | F: 5’- GGGGTGTTTCTCCGACAACT -3’ R: 5’- GGAGGTCTCTCTTTGGTGGC -3’ | 59 | NM_001106398.1 | |
Internal control | GAPDH | F: 5’- TCCCTCAAGATTGTCAGCAA -3’ R: 5’- AGATCCACAACGGATACATT -3’ | 58 | NM_017008.4 |