Fig. 3

Sumoylation of IGF-1R inhibited osteogenic differentiation of PDLSCs. a The level of IGF-1R and SNAI2 were detected by Western Blot in membrane protein and nuclear protein after 7 days of osteogenic induction. Na⁺–K⁺-ATPase and PCNA as an internal reference. And Quantitative analysis of expression levels (***p < 0. 001; ****p < 0. 0001). b The expression of IGF-1R (green) and SNAI2 (red) in PDLSCs were examined by cytofluorescence. Nuclei are stained with DAPI (blue). Scale bar 20 μm. c IGF-1R was conjugated with SNAI2 in PDLSCs was detected by immunoprecipitation (IP) with anti-IGF-1R antibody followed by Western Blot with anti-SNAI2 antibody. All results were obtained from at least three independent experiments