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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Single cell Raman spectroscopy to identify different stages of proliferating human hepatocytes for cell therapy

Fig. 4

The peak area were semi-quantitative to compare differences of the specific Raman bands a 480 \({\mathrm{cm}}^{-1}\) (glycogen), b 831 \({\mathrm{cm}}^{-1}\) (tyrosine), c 840–860 \({cm}^{-1}\)(polysaccharide structure), d 1003 \({\mathrm{cm}}^{-1}\) (phenylalanine), e 1080 \({\mathrm{cm}}^{-1}\) (amide II, typical phospholipid), f 1172 \({\mathrm{cm}}^{-1}\) (C–H in-plane bending mode of tyrosine), g 1206 \({\mathrm{cm}}^{-1}\) (hydroxyproline, tyrosine), h 1265 \({\mathrm{cm}}^{-1}\) (α-helix, collagen, tryptophan), i 1300 \({\mathrm{cm}}^{-1}\) (lipids), j 1337 \({\mathrm{cm}}^{-1}\) (amide III), k 1440 \({\mathrm{cm}}^{-1}\) (lipids), l 1658 \({\mathrm{cm}}^{-1}\) (amide I), m 1744 \({\mathrm{cm}}^{-1}\) (carbonyl feature of lipid spectra) in PHH (Lot:005), ProliHHs P1 and P4. The results represent median, ns p\(\ge\) 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 (PHH: primary human hepatocytes, ProliHHs: proliferating human hepatocytes, P1: passage 1, P4: passage 4)

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