Skip to main content
Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Valproic acid promotes the in vitro differentiation of human pluripotent stem cells into spermatogonial stem cell-like cells

Fig. 2

Gene expression and the SSCLC induction efficiency during SSCLC induction. A The percentage of PLZF+ cells at different days of differentiation detected by flow cytometry, n = 3. B Immunostaining of PLZF (green), GPR125 (red), GFRα1 (red), VASA (green) and PIWIL2 (red) of differentiated cells using developed SSCLC induction protocol at different days of differentiation, and the nuclei were stained with DAPI (blue). C The expression of genes related to SSC, meiosis and pluripotency at different days of differentiation detected by RT-qPCR, n = 3

Back to article page