Fig. 5

Differentiation of hiPSCs from NOA patients into SSCLCs. A The percentage of PLZF+ cells of 1106 and 1122 NOA patients at 12 days of differentiation detected by flow cytometry. B Immunostaining of PLZF (green) and GPR125 (red) at 12 days of differentiation, and the nuclei were stained with DAPI (blue). C The expression of genes related to SSC and Wnt signaling pathway at 12 days of differentiation detected by RT-qPCR, n = 3