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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Synergistic stimulation of osteoblast differentiation of rat mesenchymal stem cells by leptin and 25(OH)D3 is mediated by inhibition of chaperone-mediated autophagy

Fig. 2

Leptin synergistically with 25(OH)D3 to induce osteoblast differentiation of rBMMSCs. a The effects of leptin or 25(OH)D3 on rBMMSCs proliferation at 24 h, 48 h, and 72 h; b relative mRNA expression of osteogenic genes (ALP, COL1A1, RUNX2, OSX, and OSP) at 12 h of osteoblast differentiation; c western blot bands of proteins of RUNX2 and COL1A1 at day 3 of rBMMSCs osteoblast differentiation; d relative quantitative analysis of proteins of RUNX2 and COL1A1; e results of ALP staining of osteoblast differentiation of rBMMSCs at day 7; f relative ALP activity fold of osteoblast differentiation of rBMMSCs at day 7; g the synthesis of 1α,25(OH)2D3 by rBMMSCs with ODM at 24 h; h results of ARS staining of osteoblast differentiation of rBMMSCs at day 21; i relative quantitative analysis of ARS of osteoblast differentiation of rBMMSCs at day 21. (Scale bars = 200 μm. *P < 0.05, **P < 0.01, ***P < 0.001 in comparison with the control group.)

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