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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Synergistic stimulation of osteoblast differentiation of rat mesenchymal stem cells by leptin and 25(OH)D3 is mediated by inhibition of chaperone-mediated autophagy

Fig. 3

Leptin inhibited CMA of rBMMSCs by activating PI3K/AKT signal pathway. a Relative mRNA expression of CMA-related genes (LAMP2A and HSC70) of rBMMSCs in growth medium at 8 h; b western blot bands of proteins of LAMP2A and HSC70 of rBMMSCs in growth medium at 24 h; c relative quantitative analysis of proteins of LAMP2A and HSC70; d 25(OH)D3 with or without leptin did not affect the relative mRNA expression of LAMP2A and HSC70 in ODM at 8 h; e 25(OH)D3 did not affect the expression of proteins of LAMP2A and HSC70 in ODM with or without leptin at 24 h; f relative quantitative analysis of proteins of LAMP2A and HSC70; g western blot analysis of JAK2/STAT3, PI3K/AKT, and MAPK/p38 in ODM at 1 h; h relative quantitative analysis of related signal pathway proteins. (*P < 0.05, **P < 0.01, ***P < 0.001 in comparison with the control group.)

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Stem Cell Research & Therapy

ISSN: 1757-6512