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Table 1 Sequences of primers

From: Synergistic stimulation of osteoblast differentiation of rat mesenchymal stem cells by leptin and 25(OH)D3 is mediated by inhibition of chaperone-mediated autophagy

Gene

Forward (5′–3′)

Reverse (5′–3′)

Megalin

ACAACTCGGATGAACGGGAC

AGTAGGTGCCGTTGGGAAAG

ALP

AGATGGATGAGGCCATCGGA

CCAAACGTGAAAACGTGGGA

COL1A1

CACTGCAAGAACAGCGTAGC

AAGTTCCGGTGTGACTCGTG

RUNX2

CAGACCAGCAGCACTCCATA

AGACTCATCCATTCTGCCGC

OSX

GGTCCTGGCAACACTCCTAC

AAGAGGTGGGGTGCTGGATA

OSP

GAGACCATGCAGAGAGCGAG

TTGACCTCAGTCCGTAAGCC

LAMP2A

AAGAGCAGGTGGTTTCCGTG

ATGGGCACAAGGAAGTTGTCT

HSC70

CTCCATTACCCGTGCTCGAT

GAACCACCCACCAGGACAAT

LepR

TTGGTCCTCTTCTGGAGC

CCCCTTGTGGAATCTGGAGTG

β-actin

CTCTGTGTGGATTGGTGGCT

CGCAGCTCAGTAACAGTCCG

  1. ALP alkaline phosphatase, COL1A1 collagen type I, RUNX2 runt-related transcription factor 2, OSX osterix, OSP osteopontin, LAMP2A lysosomal-associated membrane protein 2A, HSC70 heat-shock cognate 71 kDa protein, LepR leptin receptor
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Stem Cell Research & Therapy

ISSN: 1757-6512