Fig. 8

Neutrophil activation and MSC survival in co-culture with neutrophils at time points 6 h (a, c) and 12 h (b, d). Ratios of 1 MSC/1 neutrophil, 1MSC/10 neutrophils and 1 MSC/100 neutrophils and control MSCs (no neutrophils) are shown. At time 6 h, universal blood donor and MHC II-high co-cultures had ratios where activation was significantly greater than the autologous co-culture. At time 12 h, only the MCH II-high co-cultures had greater activation as compared to the universal blood donor (BD) co-cultures. Median MSC survival was greater than 95% when co-cultured with neutrophils at time point 6 h (c) and 12 h (d). The only group with significant viability loss with neutrophils as compared to MSCs cultured alone was the MHC II-high group at 12 h