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Table 3 Lymphocyte activation during co-culture with MSCs

From: Immune response to allogeneic equine mesenchymal stromal cells

 

Day 3

Day 5

1:1

1:10

1:100

Control

1:1

1:10

1:100

Control

Autologous

4795

(986)a

2092

(109)a

4973

(86)a,b

–

16,435

(7688)a,#

8349

(272)a,#

8473 (8292)a,b

–

Blood donor

6882

(3570)a,#

2449

(494)a

4319

(1162)a

–

11,156

(12,090)a,b

5818

(1572)a

15,215 (7230)a,#

–

MHC II-high

9986

(6166)a,#

4996

(2114)b

7805

(5145)b,#

–

11,135

(3348)a,b

7416

(1110)a,#

7241 (2015)b

–

MHC II-low

5380

(4573)a

2202

(317)a

3800

(2815)a

–

7686

(3961)b

5787

(1819)a

9493 (5314)a,b

–

PBMC

–

–

–

1251

(226)

–

–

–

4048 (2513)

Activated PBMC

–

–

–

20,237

(27,350)

–

–

–

25,342 (29,759)

  1. Median percent of lymphocytes activated and IQR is shown for co-cultures with the ratio of MSC/PBMC and for control cultures with PBMCs cultured alone with or without activating agent. Values within each column which have different letters are significantly different (p < 0.05). Values that are significantly greater than the PBMC alone are shown with a #. Autologous, autologous MSC co-culture (n = 1); blood donor, universal blood donor MSC co-culture (n = 3); MHC II high, MHC II-high MSC co-culture (n = 3); MHC II low, MHC II-low MSC co-culture (n = 3); PBMCs, PBMCs alone; activated PBMCs, PBMCs activated with 2.5 µg/ml of PWM