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Table 5 Anabolic gene expression in MSCs co-cultured with PBMCs

From: Immune response to allogeneic equine mesenchymal stromal cells

   Autologous Blood donor MHC II-high MHC II-low
CD59
Day 3
1:1 3.53 (0.69)a 4.24 (3.08)a 5.42 (2.69)a,b 14.5 (10.4)b
Control 3.68 (0.98) 4.55 (4.52) 7.75 (7.03) 2.31 (1.08)
CD59
Day 5
1:1 9.54 (27.5) 11.5 (10.1) 5.18 (7.53) 9.08 (4.61)
Control 4.41 (0.77) 2.60 (1.90) 4.74 (2.69) 4.01 (2.08)
FGF-2
Day 3
1:1 852 (21.8)a,c 5910 (5300)b 450 (112)a 2470(2150)b,c
Control 919 (192) 5100 (3690) 955 (1110) 4150 (5550)
FGF-2
Day 5
1:1 1450 (307)a 1390 (1260)a 473 (397)b 1100 (451)a,c
Control 1100 (1040) 3570 (3590) 1820 (1520) 2850 (6170)
HGF
Day 3
1:1 41.2 (3.55)a 307 (137)b 76.4 (64.4)c 386 (184)b
Control 166 (43.4) 263 (391) 136 (48.9) 572 (440)
HGF
Day 5
1:1 85.8 (17.5) 132 (55.0) 113 (52.3) 119 (14.9)
Control 189 (80.5) 296 (236) 356 (172) 1050 (519)
IDO
Day 3
1:1 123 (26.8)a 3850 (1220)b 207 (154)a 3510 (2170)b
Control 3.90 (1.41) 8.01 (5.27) 45.7 (51.1) 9.90 (11.3)
IDO
Day 5
1:1 485 (222)a 1420 (1100)b 205 (225)a 2050 (1900)b
Control 2.16 (1.42) 4.55 (8.86) 5.08 (4.62) 3.29 (2.22)
IL-10
Day 3
1:1 17.0 (1.45)a 57.1 (88.4)b 32.0 (7.3)a 66.4 (45.5)b
Control 1.71 (1.45) 4.32 (1.55) 16.5 (12.9) 24.7 (22.1)
IL-10
Day 5
1:1 29.7 (13.5) 258 (214) 62.4 (17.6) 207 (230)
Control 5.83 (2.50) 6.71 (3.10) 5.74 (4.76) 7.15 (4.08)
IL-ra
Day 3
1:1 27.0 (3.0)a,b 76.0 (353)b,c 24.4 (14.5)a 725 (500)c
Control 2.0 (6.5) 5.7 (3.9) 14.9 (39.3) 18.5 (21.2)
IL-ra
Day 5
1:1 78.4 (33.6)a,b 65.6 (39.2)a,b 29.6 (14.6)a 106 (53.9)b
Control 3.2 (2.1) 6.4 (2.3) 5.1 (7.9) 3.2 (2.6)
IL-2
Day 3
1:1 4.7 (0.4)a 25.5 (12.3)a,b 10.7 (15.9)a 38.4 (11.6)b
Control 2.3 (0.5) 3.3 (1.1) 7.8 (1.6) 5.9 (1.3)
IL-2
Day 5
1:1 4.6 (5.2) 11.3 (9.2) 18.5 (4.7) 11.9 (5.6)
Control 2.2 (0.7) 2.5 (3.0) 1.9 (1.4) 6.0 (4.5)
Sox2
Day 3
1:1 1.0 (0.1)a,c 3.6 (2.9)b 1.0 (0)c 2.5 (2.7)a,b
Control 1.0 (0.2) 1.2 (0.3) 3.7 (10.6) 1.1 (0.2)
Sox2
Day 5
1:1 5.7 (9.7)a,b 1.0 (0.6)a,b 1.9 (2.4)a 1.0 (0)b
Control 1.0 (0) 1.0 (0) 1.0 (0) 1.0 (0)
TGFB1
Day 3
1:1 299 (20)a 625 (86)b 334 (89)a 556 (294)b
Control 422 (71) 255 (54) 283 (112) 249 (46)
TGFB1
Day 5
1:1 411 (101) 601 (320) 460 (213) 669 (384)
Control 575 (38) 428 (47) 573 (174) 409 (36)
VEGF
Day 3
1:1 19,900 (500) 20,700 (19,700) 26,100 (6600) 21,600 (4460)
Control 2880 (550) 6030 (3380) 3200 (4900) 8170 (7780)
VEGF
Day 5
1:1 18,200 (4600) 18,100 (14,600) 23,000 (6800) 14,900 (3570)
Control 4130 (406) 4410 (1650) 5520 (780) 3930 (5490)
  1. Median RNA copy number and IQR is shown for co-cultures with PBMCs and for MSCs cultured alone (control) at Days 3 and 5 of co-culture. Values for co-cultured MSC gene expression (within each row) which are significantly different are marked with different letters (p < 0.05). Autologous, autologous MSC co-culture (n = 1); blood donor, universal blood donor MSC co-culture (n = 3); MHC II high, MHC II-high MSC co-culture (n = 3); MHC II low, MHC II-low MSC co-culture (n = 3)