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Table 5 Anabolic gene expression in MSCs co-cultured with PBMCs

From: Immune response to allogeneic equine mesenchymal stromal cells

  

Autologous

Blood donor

MHC II-high

MHC II-low

CD59

Day 3

1:1

3.53 (0.69)a

4.24 (3.08)a

5.42 (2.69)a,b

14.5 (10.4)b

Control

3.68 (0.98)

4.55 (4.52)

7.75 (7.03)

2.31 (1.08)

CD59

Day 5

1:1

9.54 (27.5)

11.5 (10.1)

5.18 (7.53)

9.08 (4.61)

Control

4.41 (0.77)

2.60 (1.90)

4.74 (2.69)

4.01 (2.08)

FGF-2

Day 3

1:1

852 (21.8)a,c

5910 (5300)b

450 (112)a

2470(2150)b,c

Control

919 (192)

5100 (3690)

955 (1110)

4150 (5550)

FGF-2

Day 5

1:1

1450 (307)a

1390 (1260)a

473 (397)b

1100 (451)a,c

Control

1100 (1040)

3570 (3590)

1820 (1520)

2850 (6170)

HGF

Day 3

1:1

41.2 (3.55)a

307 (137)b

76.4 (64.4)c

386 (184)b

Control

166 (43.4)

263 (391)

136 (48.9)

572 (440)

HGF

Day 5

1:1

85.8 (17.5)

132 (55.0)

113 (52.3)

119 (14.9)

Control

189 (80.5)

296 (236)

356 (172)

1050 (519)

IDO

Day 3

1:1

123 (26.8)a

3850 (1220)b

207 (154)a

3510 (2170)b

Control

3.90 (1.41)

8.01 (5.27)

45.7 (51.1)

9.90 (11.3)

IDO

Day 5

1:1

485 (222)a

1420 (1100)b

205 (225)a

2050 (1900)b

Control

2.16 (1.42)

4.55 (8.86)

5.08 (4.62)

3.29 (2.22)

IL-10

Day 3

1:1

17.0 (1.45)a

57.1 (88.4)b

32.0 (7.3)a

66.4 (45.5)b

Control

1.71 (1.45)

4.32 (1.55)

16.5 (12.9)

24.7 (22.1)

IL-10

Day 5

1:1

29.7 (13.5)

258 (214)

62.4 (17.6)

207 (230)

Control

5.83 (2.50)

6.71 (3.10)

5.74 (4.76)

7.15 (4.08)

IL-ra

Day 3

1:1

27.0 (3.0)a,b

76.0 (353)b,c

24.4 (14.5)a

725 (500)c

Control

2.0 (6.5)

5.7 (3.9)

14.9 (39.3)

18.5 (21.2)

IL-ra

Day 5

1:1

78.4 (33.6)a,b

65.6 (39.2)a,b

29.6 (14.6)a

106 (53.9)b

Control

3.2 (2.1)

6.4 (2.3)

5.1 (7.9)

3.2 (2.6)

IL-2

Day 3

1:1

4.7 (0.4)a

25.5 (12.3)a,b

10.7 (15.9)a

38.4 (11.6)b

Control

2.3 (0.5)

3.3 (1.1)

7.8 (1.6)

5.9 (1.3)

IL-2

Day 5

1:1

4.6 (5.2)

11.3 (9.2)

18.5 (4.7)

11.9 (5.6)

Control

2.2 (0.7)

2.5 (3.0)

1.9 (1.4)

6.0 (4.5)

Sox2

Day 3

1:1

1.0 (0.1)a,c

3.6 (2.9)b

1.0 (0)c

2.5 (2.7)a,b

Control

1.0 (0.2)

1.2 (0.3)

3.7 (10.6)

1.1 (0.2)

Sox2

Day 5

1:1

5.7 (9.7)a,b

1.0 (0.6)a,b

1.9 (2.4)a

1.0 (0)b

Control

1.0 (0)

1.0 (0)

1.0 (0)

1.0 (0)

TGFB1

Day 3

1:1

299 (20)a

625 (86)b

334 (89)a

556 (294)b

Control

422 (71)

255 (54)

283 (112)

249 (46)

TGFB1

Day 5

1:1

411 (101)

601 (320)

460 (213)

669 (384)

Control

575 (38)

428 (47)

573 (174)

409 (36)

VEGF

Day 3

1:1

19,900 (500)

20,700 (19,700)

26,100 (6600)

21,600 (4460)

Control

2880 (550)

6030 (3380)

3200 (4900)

8170 (7780)

VEGF

Day 5

1:1

18,200 (4600)

18,100 (14,600)

23,000 (6800)

14,900 (3570)

Control

4130 (406)

4410 (1650)

5520 (780)

3930 (5490)

  1. Median RNA copy number and IQR is shown for co-cultures with PBMCs and for MSCs cultured alone (control) at Days 3 and 5 of co-culture. Values for co-cultured MSC gene expression (within each row) which are significantly different are marked with different letters (p < 0.05). Autologous, autologous MSC co-culture (n = 1); blood donor, universal blood donor MSC co-culture (n = 3); MHC II high, MHC II-high MSC co-culture (n = 3); MHC II low, MHC II-low MSC co-culture (n = 3)