Skip to main content

Table 6 Catabolic gene expression in MSCs co-cultured with PBMCs

From: Immune response to allogeneic equine mesenchymal stromal cells

  

Autologous

Blood donor

MHC II-high

MHC II-low

ADAMTS-4

Day 3

1:1

179 (13)a

924 (997)b,c

371 (137)a,c

526 (282)c

Control

66 (30)

28 (20)

90 (44)

34 (8)

ADAMTS-4

Day 5

1:1

253 (50)a

882 (444)b,c

1087 (805)b

671 (606)a,c

Control

108 (27)

85 (21)

117 (183)

50 (25)

ADAMTS-5

Day 3

1:1

2050 (220)a

5110 (3940)b

2420 (610)a

4000 (2200)a,b

Control

1150 (150)

2200 (780)

1390 (1240)

1260 (910)

ADAMTS-

Day 5

1:1

6990 (1450)a

2490 (3840)a,b

2950 (2040)a

1540 (110)b

Control

1210 (260)

2230 (680)

1680 (1270)

835 (1170)

CCL2

Day 3

1:1

14,200 (1300)a

59,900 (25,400)b

15,300 (8800)a

76,600 (60,000)b

Control

2110 (330)

4080 (4230)

2840 (1140)

4110 (3470)

CCL2

Day 5

1:1

10,100 (1700)a,c

38,200 (11,400)b

3440 (4060)a

36,900 (19,300)a,c

Control

1820 (100)

2880 (2230)

2190 (830)

2760 (1860)

IL-8

Day 3

1:1

860 (66)a

6570 (22,200)b,c

2770 (1460)a,c

12,800 (17,800)b,c

Control

10.9 (5.4)

146 (234)

9.88 (10.0)

32.2 (30.7)

IL-8

Day 5

1:1

66.2 (95.3)a

2370 (3930)b

176 (472)a

3140 (8670)b

Control

2.64 (1.38)

97.7 (70.1)

9.6 (6.5)

6.31 (12.7)

IFNÝ

Day 3

1:1

9.77 (3.88)a

392 (164)b

6.29 (10.8)a

492 (359)b

Control

2.20 (0.53)

5.66 (6.84)

20.8 (28.2)

3.22 (1.27)

IFNÝ

Day 5

1:1

21.5 (21.0)a,c

546 (618)b

13.4 (10.5)a

640 (680)a,c

Control

2.41 (3.20)

2.78 (2.59)

2.25 (1.35)

3.93 (3.53)

IL-1b

Day 3

1:1

12.4 (6.3)a

255 (1050)b,c

47.1 (42.2)a,b

460 (1457)c

Control

3.95 (1.53)

11.9 (3.5)

5.3 (11.4)

12.6 (9.8)

IL-1b

Day 5

1:1

32.1 (23.0)a

230 (279)b

22.4 (11.7)a

352 (1016)b

Control

11.8 (5.0)

13.8 (4.4)

8.15 (9.35)

6.15 (4.16)

IL-6

Day 3

1:1

16,200 (800)a,b

50,200(24,400)a

6240 (3310)b

47,500 (56,200)b

Control

48.9 (14.6)

1360 (1100)

40.5 (52.2)

234 (460)

IL-6

Day 5

1:1

4750 (1520)a,b

19,500 (9200)a

1010 (2090)b

13,400 (6000)a

Control

33.6 (31.8)

541 (111)

24.1 (17.0

79.1 (283)

MMP-13

Day 3

1:1

315 (16)a,b

92.7 (6945)a

855 (372)b

140 (47)a

Control

18.1 (6.9)

181 (195)

1010 (8550)

368 (352)

MMP-13

Day 5

1:1

687 (181)a

136 (872)a

935 (607)a

62.8 (70.8)b

Control

90.3 (19.2)

242 (225)

2190 (14,500)

1010 (1040)

COX2

Day 3

1:1

9400 (810)a,b

16,400 (7900)a

7610 (2820)b

9440 (18,700)a

Control

71.5 (10.3)

3560 (4100)

344 (1350)

1690 (1520)

COX2

Day 5

1:1

8930 (4150)

10,300 (8900)

6290 (26,700)

4990 (1850)

Control

186 (1220)

6830 (6520)

558 (1280)

612 (1690)

TNFa

Day 3

1:1

8.29 (3.65)a

48.9 (17.5)b

15.1 (8.5)a

51.8 (42.1)b

Control

2.17 (1.27

6.22 (1.97)

15.2 (10.8)

14.2 (3.0)

TNFa

Day 5

1:1

29.8 (31.9)

52.1 (44.3)

12.2 (23.9)

63.3 (35.5)

Control

1.77 (0.71)

3.65 (0.99)

5.45 (2.32)

4.61 (1.19)

  1. Median RNA copy number and IQR is shown for co-cultures with PBMCs and for MSCs cultured alone (control) at Days 3 and 5 of co-culture. Values for co-cultured MSC gene expression (within each row) which are significantly different are marked with different letters (p < 0.05). Autologous, Autologous MSC co-culture (n = 1); blood donor, universal blood donor MSC co-culture (n = 3); MHC II high, MHC II-high MSC co-culture (n = 3); MHC II Low, MHC II-low MSC co-culture (n = 3)