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Table 6 Catabolic gene expression in MSCs co-cultured with PBMCs

From: Immune response to allogeneic equine mesenchymal stromal cells

   Autologous Blood donor MHC II-high MHC II-low
ADAMTS-4
Day 3
1:1 179 (13)a 924 (997)b,c 371 (137)a,c 526 (282)c
Control 66 (30) 28 (20) 90 (44) 34 (8)
ADAMTS-4
Day 5
1:1 253 (50)a 882 (444)b,c 1087 (805)b 671 (606)a,c
Control 108 (27) 85 (21) 117 (183) 50 (25)
ADAMTS-5
Day 3
1:1 2050 (220)a 5110 (3940)b 2420 (610)a 4000 (2200)a,b
Control 1150 (150) 2200 (780) 1390 (1240) 1260 (910)
ADAMTS-
Day 5
1:1 6990 (1450)a 2490 (3840)a,b 2950 (2040)a 1540 (110)b
Control 1210 (260) 2230 (680) 1680 (1270) 835 (1170)
CCL2
Day 3
1:1 14,200 (1300)a 59,900 (25,400)b 15,300 (8800)a 76,600 (60,000)b
Control 2110 (330) 4080 (4230) 2840 (1140) 4110 (3470)
CCL2
Day 5
1:1 10,100 (1700)a,c 38,200 (11,400)b 3440 (4060)a 36,900 (19,300)a,c
Control 1820 (100) 2880 (2230) 2190 (830) 2760 (1860)
IL-8
Day 3
1:1 860 (66)a 6570 (22,200)b,c 2770 (1460)a,c 12,800 (17,800)b,c
Control 10.9 (5.4) 146 (234) 9.88 (10.0) 32.2 (30.7)
IL-8
Day 5
1:1 66.2 (95.3)a 2370 (3930)b 176 (472)a 3140 (8670)b
Control 2.64 (1.38) 97.7 (70.1) 9.6 (6.5) 6.31 (12.7)
IFNÝ
Day 3
1:1 9.77 (3.88)a 392 (164)b 6.29 (10.8)a 492 (359)b
Control 2.20 (0.53) 5.66 (6.84) 20.8 (28.2) 3.22 (1.27)
IFNÝ
Day 5
1:1 21.5 (21.0)a,c 546 (618)b 13.4 (10.5)a 640 (680)a,c
Control 2.41 (3.20) 2.78 (2.59) 2.25 (1.35) 3.93 (3.53)
IL-1b
Day 3
1:1 12.4 (6.3)a 255 (1050)b,c 47.1 (42.2)a,b 460 (1457)c
Control 3.95 (1.53) 11.9 (3.5) 5.3 (11.4) 12.6 (9.8)
IL-1b
Day 5
1:1 32.1 (23.0)a 230 (279)b 22.4 (11.7)a 352 (1016)b
Control 11.8 (5.0) 13.8 (4.4) 8.15 (9.35) 6.15 (4.16)
IL-6
Day 3
1:1 16,200 (800)a,b 50,200(24,400)a 6240 (3310)b 47,500 (56,200)b
Control 48.9 (14.6) 1360 (1100) 40.5 (52.2) 234 (460)
IL-6
Day 5
1:1 4750 (1520)a,b 19,500 (9200)a 1010 (2090)b 13,400 (6000)a
Control 33.6 (31.8) 541 (111) 24.1 (17.0 79.1 (283)
MMP-13
Day 3
1:1 315 (16)a,b 92.7 (6945)a 855 (372)b 140 (47)a
Control 18.1 (6.9) 181 (195) 1010 (8550) 368 (352)
MMP-13
Day 5
1:1 687 (181)a 136 (872)a 935 (607)a 62.8 (70.8)b
Control 90.3 (19.2) 242 (225) 2190 (14,500) 1010 (1040)
COX2
Day 3
1:1 9400 (810)a,b 16,400 (7900)a 7610 (2820)b 9440 (18,700)a
Control 71.5 (10.3) 3560 (4100) 344 (1350) 1690 (1520)
COX2
Day 5
1:1 8930 (4150) 10,300 (8900) 6290 (26,700) 4990 (1850)
Control 186 (1220) 6830 (6520) 558 (1280) 612 (1690)
TNFa
Day 3
1:1 8.29 (3.65)a 48.9 (17.5)b 15.1 (8.5)a 51.8 (42.1)b
Control 2.17 (1.27 6.22 (1.97) 15.2 (10.8) 14.2 (3.0)
TNFa
Day 5
1:1 29.8 (31.9) 52.1 (44.3) 12.2 (23.9) 63.3 (35.5)
Control 1.77 (0.71) 3.65 (0.99) 5.45 (2.32) 4.61 (1.19)
  1. Median RNA copy number and IQR is shown for co-cultures with PBMCs and for MSCs cultured alone (control) at Days 3 and 5 of co-culture. Values for co-cultured MSC gene expression (within each row) which are significantly different are marked with different letters (p < 0.05). Autologous, Autologous MSC co-culture (n = 1); blood donor, universal blood donor MSC co-culture (n = 3); MHC II high, MHC II-high MSC co-culture (n = 3); MHC II Low, MHC II-low MSC co-culture (n = 3)