Fig. 4

PF-127 plus SAP combination promotes engraftment of WJMSCs in diabetic wound. a–c Bright-field and fluorescence microscopy of WJMSCs stably expressing Flag-tagged EGFP was performed (A-B) and either western blotted to confirm EGFP expression with the indicated antibodies. The tubulin was shown as internal control. d Schematic of OE-EGFP WJMSCs migration by fluorescence assay at 24 h and 72 h after transplantation in ulcer wound. e, f At 24 h after transplantation, EGFP-overexpressing WJMSCs in different groups were detected (E) and the number of EGFP-positive WJMSCs was analyzed quantitatively in each group (F). Error bars represent mean ± SEM; n = 3 independent experiments. Significance was determined using one-way ANOVA. ***p < 0.001. Scale bar: 50 μm. Enlarge scale bar: 25 μm. g, h At 72 h after transplantation, EGFP-overexpressing WJMSCs in different groups were detected (g) and the number of EGFP-positive WJMSCs was analyzed quantitatively in each group (H). Error bars represent mean ± SEM; n = 3 independent experiments. Significance was determined using one-way ANOVA. ***p < 0.001. Scale bar: 50 μm. Enlarge scale bar: 25 μm