Table 1 The advantages and disadvantages of several commonly used loading methods
From: Mesenchymal stem cells-derived exosomes for drug delivery
Methods | Type of cargo | Advantages | Disadvantages | References |
|---|---|---|---|---|
Transfection | RNA | Good load efficiency and stability of the molecule | Potential toxicity and genetic changes | [36] |
Electroporation | Hydrophilic cargo | Wide applicability | Require sophisticated equipment/possible molecular aggregation/morphological changes of exosomes | |
Overexpression | Protein | Simple and convenient | May cause imbalance of cell proliferation and apoptosis |