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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Activation of adult mammalian retinal stem cells in vivo via antagonism of BMP and sFRP2

Fig. 6

Ciliary epithelium derived tdTomato+ cells in the neural retina express photoreceptor or retinal ganglion cell markers. A-L Analyses of retinal cell type markers in eye slides from the endpoint of the experimental paradigm outlined in Fig. 4A. A Quantification of the percentage of Msx1-tdTomato+ cells in the retina that co-labeled for the photoreceptor marker Recoverin in eye sections from the indicated conditions. There was a significant effect of treatment (p = 0.026) and injury (p = 0.034) on the proportion of Msx1-tdTomato+Recoverin+ cells. N = 5–8 eyes per group, 3–6 slides per eye. B-F Brightfield and fluorescence overlay images of Msx1-Cre driven tdTomato expression and immunostaining for photoreceptor marker Recoverin in eye sections from the indicated conditions. DRAQ5 stain was used to label all nuclei. White arrows indicate Msx1-tdTomato+Recoverin+ co-labeled cells. G Quantification of the percentage of Msx1-tdTomato+ cells in the retina that co-labeled for the retinal ganglion cell marker Brn3a in eye sections from the indicated conditions. There was a significant interaction between treatment and injury on the proportion of Msx1-tdTomato+Brn3a+ cells (p = 0.002; N = 5–8 eyes per group, 3–6 slides per eye). H–L Brightfield and fluorescence overlay images of Msx1-Cre driven tdTomato expression and immunostaining for retinal ganglion cell marker Brn3a in eye sections from the indicated conditions. DRAQ5 stain was used to label all nuclei. White arrows indicate Msx1-tdTomato+Brn3a+ co-labeled cells. Straight dashed line indicates the border between the ciliary epithelium (CE) and the neural retina (NR). Dashed line box indicates high magnification inset. Statistics: Two-way ANOVAs with Holm-Sidak posthoc tests. Data are means ± SEMs.* = p < 0.05

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