Fig. 5

a Normalized ALP activity detected at 10 days of osteogenic induction of USCs cultured on each scaffold. The cross-linked scaffolds containing 0%, 0.01%, 0.05%, 0.1%. 0.5%, and 1% GO were labeled “0%,” “0.01%,” “0.05%,” “0.1%,” “0.5%,” and “1%.” b–e Detection of the mRNA levels of selected osteogenic markers in USCs at 7 and 21 days of osteogenic induction. *p < 0.05 and **p < 0.01 versus the 0% group. #p < 0.05 and ##p < 0.01 versus the 0.1% group