Fig. 1

TM9SF4 deficiency promotes osteogenesis. A, B Primary MSCs and MC3T3-E1 cell lines were cultured in osteogenic medium for 7d and 5d, respectively, before qRT-PCR detection. Error bars represent mean ± SEM, n = 3 independent experiments. *P < 0.05, **P < 0.01, ns = not significant. C, D Primary MSCs were induced in osteogenic medium for 2w and MC3T3-E1 cell lines for 5d before ALP staining. Scale bars = 1 mm. E, F Calcium deposition was tested via Alizarin Red S staining after osteogenic induction, primary MSCs for 2w, MC3T3-E1 cell lines for 10d. Scale bars = 1 mm. G Knockdown efficiency of TM9SF4 in MC3T3-E1 cells was detected via Western blot. Error bars represent mean ± SEM, n = 3. **P < 0.01, ***P < 0.001 (D, F, G)