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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: TM9SF4 is a novel regulator in lineage commitment of bone marrow mesenchymal stem cells to either osteoblasts or adipocytes

Fig. 4

Canonical Wnt pathway is involved in TM9SF4-regulated osteogenesis. A Wnt ligands were examined by qRT-PCR after primary MSCs were cultured in osteogenic medium for 5d. Error bars represent mean ± SEM, n = 3. *P < 0.05, ns = not significant. B, C Primary MSCs were induced with osteogenic medium for 1w and MC3T3-E1 cells for 3d and 5d, respectively, before Western Blot detection. D, E Primary MSCs were incubated in osteogenic medium with 10 µM Wnt-C59 for 1w and MC3T3-E1 cells for 5d before RNA detection. Error bars represent mean ± SEM, n = 3. **P < 0.01, ***P < 0.001, ****P < 0.0001. F, H Cells were induced with osteogenic medium with 10 µM Wnt-C59, primary MSCs for 2w and MC3T3-E1 cells for 10d. Scale bars = 1 mm. Error bars represent mean ± SEM, n = 3. **P < 0.01, ***P < 0.001, ****P < 0.0001 (G, I)

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