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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: TM9SF4 is a novel regulator in lineage commitment of bone marrow mesenchymal stem cells to either osteoblasts or adipocytes

Fig. 5

TM9SF4 suppression promotes actin filament polymerization. Actin fibers were stained with Alexa Fluor 488-phalloidin and observed under confocal microscope with 63 × oil immersion objective lens. A Confocal images of F-actin in primary MSCs and stable MC3T3-E1 cell lines. Scale bars = 50 µm. B Transfection efficiency was detected via qRT-PCR in TM9SF4−/− MSCs after rescue with pcDNA6-TM9SF4-V5. Error bars represent mean ± SEM, n = 3. ****P < 0.0001. C Representative images of F-actin (green) and TM9SF4-V5 (red) in rescued MSCs. Scale bars = 50 µm

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Stem Cell Research & Therapy

ISSN: 1757-6512