Fig. 6

Cytochalasin D suppresses osteogenesis by disrupting actin fibers. A, B Confocal images of actin fibers in primary MSCs and ME3T3-E1 cell lines after treated with 0.1 µg/ml CytoD for 24 h. Scale bars = 50 µm. C, D Osteogenic markers were detected by qRT-PCR after induced with osteogenic medium, primary MSCs for 1w, MC3T3-E1 cells for 5d. Error bars represent mean ± SEM, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns = not significant. E–H Alizarin Red S staining after osteogenesis, primary MSCs for 2w, MC3T3-E1 cells for 10d. Scale bars = 1 mm. Error bars represent mean ± SEM, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns = not significant (F, H)