Fig. 4

Immunofluorescence analysis of chondrogenesis-associated proteins. Immunofluorescence labeling and quantitative analysis indicate that chondrogenic medium dramatically improved expressions of chondrogenesis-associated proteins (including Col2, Aggrecan, and SOX9) and EMF could further enhance their intensities. Data are shown as mean ± standard deviation (n = 5). # indicates no significant difference, *p < 0.05, **p < 0.01, ***p < 0.001 compared to group without EMF