Fig. 3

MTS assay shows there is no difference in the viability of hES-derived VM progenitor cells plated on plastic (open circles) or cellulose. Values represent absorbance at 490 nm as normalised to laminin coated plastic (Lam-wells). The functionalisation method requiring covalent binding of peptides to cellulose has no negative effects on the cell metabolism, as seen from MTS assay data obtained for functionalised laminin + BDNF + GDNF (Lam-GFs-cov) or functionalised laminin only (Lam-cov); one-way analysis of variance (ANOVA); P = 0.7883; F (3, 8) = 0.3532. Tukey’s multiple comparison P values (Laminin (A), cellulose + laminin (B), cellulose + functionalised laminin (C), cellulose + functionalised GFs (D): (A–B) not significant (NS) = 0.8655; (A–C) NS = 0.8634; (A–D) NS ≥ 0.9996; (B–C) NS ≥ 0.9999; (B–D) NS = 0.9041; (C–D) NS = 0.9023). Experiments were performed from N = 3 experimental repeats with n = 3 technical repeats. Mean values ± standard deviation are plotted