Fig. 4

hES-derived VM progenitors grown on bacterial cellulose express markers of dopaminergic lineage. VM progenitors grown on laminin coated bacterial cellulose (Lam-coat) express FOXA2, LMX1, and FOXA2 and are colocalised with LMX1. The expression of FOXA2 is comparable to the plastic substrate controls (Lam-wells). The expression of LMX1 and LMX1 with FOXA2 is decreased compared with cells cultured on plastic. The proportion of cells showing colocalisation of a nuclear marker, Hoechst 33,342 (blue) with the transcription factor A FOXA2 (magenta); B LMX1 (yellow); C FOXA2 (magenta) and LMX1 (yellow). Two-tailed unpaired Student’s t test were used for statistical analysis. ***P < 0.001. Experiments were performed from N = 3 experimental repeats with n = 3 technical repeats. All data are plotted with mean values ± standard deviation normalised to Laminin coated plastic (Lam-wells). Scale bar: 30 µm