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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Biofunctionalised bacterial cellulose scaffold supports the patterning and expansion of human embryonic stem cell-derived dopaminergic progenitor cells

Fig. 5

Growth of stem cells on GF-functionalised bacterial cellulose further increases the expression of FOXA2, LMX1, and FOXA2 colocalised with LMX1 compared to laminin only coated cellulose samples. The percentage of cells showing co-localisation of a nuclear marker, Hoechst 33,342 (blue) with the transcription factor A FOXA2 (magenta); B LMX1 (yellow); C FOXA2 (magenta) and LMX1 (yellow), as determined by manual counting. A (one-way analysis of variance (ANOVA); P = 0.1090; F (2, 43) = 2.145). Tukey’s multiple comparison P values [Lam-coat (A), Lam-cov (B), Lam-GFs-cov (C)]: A, B not significant (NS) = 0.8503; AC NS = 0.1065; B, C NS = 0.2894. B (one-way analysis of variance (ANOVA); *P = 0.0116; F (2, 40) = 1.961) Tukey’s multiple comparison P values [Lam-coat (A), Lam-cov (B), Lam-GFs-cov (C)]: A, B not significant (NS) = 0.1200; AC **P = 0.0093; B, C NS = 0.4905. C (one-way analysis of variance (ANOVA); **P = 0.0044; F (2, 34) = 2.152). Tukey’s multiple comparison P values [Lam-coat (A), Lam-cov (B), Lam-GFs-cov (C)]: A, B not significant (NS) = 0.1200; AC **P = 0.0030; B, C NS = 0.2250

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