Fig. 5

Myeloid lineage analysis in mice after NLRP3 gene knockout. A Gene expression of SCF in wild-type (WT) and NLRP3^−/− (KO) BA-MSCs by qRT-PCR (n = 4), and ELISA analysis in the supernatants after culture for 7 days (n = 3). B Gene expression of CXCL12 in WT and NLRP3^−/− BA-MSCs by qRT-PCR (n = 4), and ELISA analysis in the supernatants after culture for 7 days (n = 3). C Gene expression of G-CSF, M-CSF, GM-CSF, and IL-6 in WT and NLRP3^−/− BA-MSCs by qRT-PCR (n = 4). D Analysis of peripheral blood counts of red blood cells (RBC), white blood cells (WBC) and platelets (PLT) in WT and NLRP3 KO mice (n = 3). E Flow cytometry analysis of the percentage of CD11b⁺/Gr-1⁺ myeloid cells in the peripheral blood of WT and NLRP3 KO mice (n = 3). F Spleen from WT and NLRP3 KO mice and analysis of spleen index (the ratio of spleen/body weight) in WT and NLRP3 KO mice (n = 6). G Flow cytometry analysis of the percentage of CD11b⁺/Gr-1⁺ myeloid cells in the spleen of WT and NLRP3 KO mice (n = 3). H Flow cytometry analysis of the percentage of LSK cells and the number of total cells, LSK, LT-HSC, ST-HSC, and MPP in the bone marrow of WT and NLRP3 KO mice (n = 5). All data are presented as mean ± SEM. ns: not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001