Fig. 6

Cell cycle and apoptosis analysis of HSPCs after co-culture with NLRP3^−/− BA-MSCs. (A-B) The co-culture of HSPCs from wild-type (WT) mice and BA-MSCs from NLRP3^−/− (KO) mice was performed in MEM-alpha medium. After 48 h, suspending HSPCs were harvested for analysis. Ctrl: HSPCs cultured alone; KO: HSPCs cultured with NLRP3^−/− BA-MSCs; WT: HSPCs cultured with WT BA-MSCs. A Flow cytometry analysis of the percentage of LT-HSC, ST-HSC, MPP, CMP, and GMP after co-culture (n = 3). B Flow cytometry analysis of the percentage of CD11b⁺/Gr-1⁺ myeloid cells after co-culture (n = 3). C Cell cycle analysis of HSPCs after co-culture with WT and NLRP3^−/− BA-MSCs (n = 3). D Apoptosis analysis of HSPCs after co-culture with WT and NLRP3^−/− BA-MSCs (n = 3). All data are presented as mean ± SEM. ns: not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001