Fig. 3

Cell seeding in scaffold-hydrogel constructs. a Distribution of 2D and 3D BMSC in constructs after seeding, scale bars 100 µm. c Representative confocal images showing cell viability based on the live (green) and dead (red) assay after 1, 7 and 21 days; corresponding 3D z-stack views of constructs at 21 days showing cell spreading on and/or in between the scaffold filaments; dotted lines indicate outlines of the printed filaments; scale bars 200 µm