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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Nanog/NFATc1/Osterix signaling pathway-mediated promotion of bone formation at the tendon–bone interface after ACL reconstruction with De-BMSCs transplantation

Fig. 1

Characterization of rabbit-derived BMSCs and De-BMSCs. a Schematic diagram illustrating the procedure for generating De-BMSCs. BMSCs that underwent osteogenic differentiation, dedifferentiation, and redifferentiation are shown. b Cell surface markers of BMSCs and De-BMSCs. c Alkaline phosphatase (ALP), oil red O, and safranin O staining to detect osteogenesis (×40), adipogenesis (×100), and chondrogenesis (×100), respectively, of BMSCs and De-BMSCs. d Cell proliferation ability of BMSCs and De-BMSCs was detected by CCK-8 at 1d, 3d, 5d, and 7d (n = 6). *p < 0.05, **p < 0.01. OIM osteogenic induction media, BMSCs bone marrow mesenchymal stem cells, Os-BMSCs osteogenic bone marrow mesenchymal stem cells, De-BMSCs dedifferentiated osteogenic bone marrow mesenchymal stem cells, Re-Os-BMSCs redifferentiation osteogenic bone marrow mesenchymal stem cell

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Stem Cell Research & Therapy

ISSN: 1757-6512