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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Nanog/NFATc1/Osterix signaling pathway-mediated promotion of bone formation at the tendon–bone interface after ACL reconstruction with De-BMSCs transplantation

Fig. 2

The osteogenic differentiation ability of De-BMSCs cultured for different dedifferentiation times. a Staining with alizarin red S (×100) and staining for alkaline phosphatase (×40) to detect the osteogenic differentiation of De-BMSCs. b mRNA expression of osteoblast marker genes, including COL1A1, OCN, and OPN. c Protein expression of osteoblast marker genes, including COL1A1, OCN, and OPN. Data represent the mean ± SD (n = 3). *p < 0.05, **p < 0.01. BMSCs, bone marrow mesenchymal stem cells; De-BMSCs dedifferentiated osteogenic bone marrow mesenchymal stem cells, COL1A1 α1 chain of type I collagen, OCN osteocalcin, OPN osteopontin

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Stem Cell Research & Therapy

ISSN: 1757-6512