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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: HO-1/BMMSC perfusion using a normothermic machine perfusion system reduces the acute rejection of DCD liver transplantation by regulating NKT cell co-inhibitory receptors in rats

Fig. 2

HO-1/BMMSCs perfused by NMP reduced the ACR after LT. A Histopathology in the liver of each group after LT. After 7 days of LT, the liver tissue of the NMP group in the SCS group was significantly damaged, in which a large number of liver cells around the vein had disappeared, and a large number of macrophage and lymphocyte infiltration, liver tissue damage in HMP and FK506 groups was mild, with a small amount of lymphocyte infiltration. B TUNEL staining of the liver tissue after LT. C Statistical results of the number of TUNEL+ cells in each group after LT. The TUNEL + cells in the HMP group and FK506 group were significantly lower than those in the other operation groups, and there was no significant difference between the HMP group and FK506 group. D The RAI score evaluates the degree of ACR after LT. CD3 (green) immunofluorescent staining (E) to show lymphocytes (indicated by the white arrow) and CD68 (red) immunofluorescent staining (F) to show macrophages (indicated by the white arrow). Statistical results for the number of CD3+ (G) cells and CD68+ (H) cells in each group after LT. I Survival analysis of recipient rats after LT. The median survival time of the Sham, SCS, NMP, BMP, HBP, and FK506 groups were > 60, 1.5, 16.5, 24.5, > 60, and > 60 d, respectively. J Rat liver function after LT. The level of ALT, AST, and TBil in the HMP and FK506 groups were significantly lower than that in the SCS and NMP groups. There was no significant difference between the HMP and FK506 groups. *P < 0.05

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