Fig. 3

VEGF expression was decreased by TRPC6-knockdown in sevoflurane preconditioned MSCs under H/R. Representative images and quantification of Western Blotting to access protein expression of TRPC6, HIF-1α, VEGF and CXCR4 in all groups (a). VEGF level in the supernatant was measured by ELISA kits (b). Data are shown as Mean ± SEM, n = 3 per group. ^*P < 0.05, ^**P < 0.01 versus siCon (negative siRNA treated MSCs under H/R), siTRPC6 groups (TRPC6-siRNA treated MSCs under H/R)