Fig. 3

Multiocular organoids were composed of neuroretina, pigmented region and corneal-like epithelium and endothelium, and conjunctiva. A Bright field and hematoxylin and eosin (HE) images of CB30-derived multiocular organoids at 90 DIV showing the following different ocular regions: neuroretina, pigmented area, and corneal-like. Scale bars: 3 mm (left image); 500 µm in bright field; 250 µm in HE. B HE and confocal images of representative multiocular organoid paraffin sections at 90 DIV consisting of pigmented epithelium (PE; bestrophin-1 (BEST)), corneal epithelium (CE; CK19 and CK5), neuroretina (NR; NRL and TUJ1), stromal cells (vimentin (VIM)), and collagen type IV (COL IV)). Scale bars: 500 µm. C HE (a, f, i) and confocal images of detailed ocular areas. NR expressed VIM, PAX6 and TUJ1 in the inner layer and recoverin (RECOV) in the outer layer (a–e), CE expressed CK3 (corneal marker) and CK19 (conjunctival marker) (g, h), and PE expressed MITF (RPE marker) and COL IV. Scale bars: 50 µm. Nuclei were stained with DAPI