Fig. 2
From: Biliary atresia-specific deciduous pulp stem cells feature biliary deficiency
Hepatic properties and functions of hepatocyte-like cells converted from BA-SHED (BA-SHED-Heps) and Cont-SHED (Cont-SHED-Heps). a The graphs show the gene expression of hepatocyte-specific genes by RT-qPCR. ALB, albumin; ARG2, arginase 2; ASL, argininosuccinate lyase; ASS1, argininosuccinate synthase 1; CPS1, carbamoyl-phosphate synthase 1; CYP3A7, cytochrome P450 3A4; CYP3A7, cytochrome P450 3A7; FAH, fumarylacetoacetate hydrolase; FASN, fatty acid synthase; GSK3B, glycogen synthase kinase 3 beta; HGF, hepatocyte growth factor; KRT18, keratin 18; NAGS, N-acetyl glutamate synthase; OTC, ornithine transcarbamylase; SREBF1, sterol regulatory element-binding transcriptional factor 1; TAT, tyrosine aminotransferase. Results are shown as ratios to 18S ribosomal RNA (/18S). b Representative images show the expression of ALB, KRT18, and cadherin 1 (CDH1) by immunofluorescent analysis. c The graphs show the amount of ALB (left panel), glucose (middle panel), and triglyceride (right panel) in the conditioned medium (CM) of cultures by ELISA and colorimetry assay. d The graph shows the xenobiotic activity of CYP3A4 under dexamethasone loading and the amount of urea, total bilirubin, and direct bilirubin in the CM by colorimetry assay. The results of CYP3A4 activity are shown as ratios to untreated cultures. e, f Representative images show uptake of Dil-fluorescent dye-conjugated acetylated low-density lipoprotein (Dil-ac-LDL) and cholyl-lysyl-fluorescein (CLF). a, c, d: n = 3 for all groups. *P < 0.05, **P < 0.01, ***P < 0.005. nd, no detection; ns, no significance. Graph bars represent means ± SEM. b, e, f: Nuclei were stained with 4', 6-diamidino-2-phenylindole (DAPI). Scale bars, 30 µm