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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Biliary atresia-specific deciduous pulp stem cells feature biliary deficiency

Fig. 4

Transplanted donor BA-SHED regenerate the parenchyma, but not the intrahepatic bile ducts in the livers of recipient CCl4-treated mice. a Representative images of in vivo kinetics of Cont-SHED and BA-SHED were detected in CCl4-treated mice 24 h after transplantation by DiR labeling. b–h Mice were harvested 8 weeks after CCl4 treatment. Representative images of human leukocyte antigen A, B, and C (HLA-ABC), hepatocyte paraffin 1 antigen (HepPar1), and human ALB (hALB) in the liver were detected by immunohistochemical staining (b). The graph shows the HepPar1-positive area in mouse livers by immunohistochemical analysis (c). The graph shows the serum levels of hALB in mice by ELISA (d). Representative images of livers were analyzed by hematoxylin and eosin (H&E) staining (e). Representative images of human KRT19 (hKRT19) in livers were detected by immunohistochemical staining. Nuclei were stained by hematoxylin (f). Representative images of hKRT19 and human mitochondria (hMt) in livers were detected by double immunofluorescent staining. Nuclei were stained by DAPI (g).The graphs show the serum levels of direct and total bilirubin in mice by colorimetry (h). a–g: Control, olive oil-treated mice; CCl4, CCl4 treated mice; CCl4 + Cont-SHED, Cont-SHED transplanted CCl4 treated mice; CCl4 + BA-SHED, BA-SHED transplanted CCl4 treated mice. b, e–g: Scale bars, 200 µm. PV, portal vein. arrowheads, biliary duct. c, d, h: n = 9 for all groups. **P < 0.01, ***P < 0.005. nd, no detection; ns, no significance. The graph bars represent the means ± SEM

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