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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Genome-wide differential expression profiling of lncRNAs and mRNAs in human induced pluripotent stem cell-derived endothelial cells exposed to e-cigarette extract

Fig. 1

Assessment of the biological effects of e-cig on iPSC-ECs. A Cell viability, B ROS production, and C caspase 3/7 activity were assessed by exposing cells to various concentrations (2–10 TPE) of menthol-flavored EAE for either 24 or 48 h. D Tube formation ability was also examined by exposing cells to 6.5 TPE EAE for 16 h to form capillary-like structure and then imaged via phase-contrast microscopy (10 x). E Representative images and quantitative data analysis of migration assays preformed on cells treated with 6.5 TPE EAE at 0, 16, and 24 h. F Permeability of ECs was measured by the transwell permeability assay with streptavidin–HRP and TMB following exposure to 6.5 TPE EAE for 24 h. G Flow cytometric data for the Annexin V and PI staining of cells treated with 6.5 TPE EAE. Data were obtained using iPSC-ECs from four healthy donors, and the assays were repeated three times. Data are represented as mean ± SD. * and ** indicate p < 0.05 and p < 0.001, respectively. Scale bars = 500 μm.

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